September 1, 1999RNAOpen Access

A selection system for functional internal ribosome entry site (IRES) elements: Analysis of the requirement for a conserved GNRA tetraloop in the encephalomyocarditis virus IRES

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Population

COS-7 mammalian cells and Escherichia coli

Comparison

Mutant encephalomyocarditis virus IRES elements vs Wild-type IRES elements and other mutant variants

Design

Preclinical

Authors

Discussion

RNRA motif may enable IRES engineering; leaves open validation in cardiac infection models before any therapeutic consideration.

Population

COS-7 mammalian cells and Escherichia coli

Intervention

Mutant encephalomyocarditis virus IRES elements (wild-type hairpin loop sequence GCGA converted to NNNN)

Comparator

Wild-type IRES elements and other mutant variants

Outcome

Functional IRES activity (translation of cell-surface-targeted protein sFv)surrogate

A conserved RNRA tetraloop consensus is required for optimal functional activity of the encephalomyocarditis virus IRES element.

Robertson et al. (1999) studied this question.

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