What question did this study set out to answer?

To examine whether functional intercellular gap junction coupling occurs between adult ventricular myocytes and myofibroblasts, and to determine how they bidirectionally affect each other's activity.

June 14, 2007ENLIGHTEN (Jurnal Bimbingan dan Konseling Islam)Open Access

Evidence of intercellular coupling between co‐cultured adult rabbit ventricular myocytes and myofibroblasts

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Population

Co-cultured adult rabbit ventricular myocytes and cardiac myofibroblasts

Comparison

Gap junction uncoupler heptanol, field… vs Control conditions

Design

Preclinical

Follow-up

24 h

Authors

LCLisa ChiltonLoyola University Medical Center WGWayne R. GilesQB3 GSGodfrey L. SmithElectrophysiology

Discussion

Member takes

Implication

May modulate myocyte electrical activity in fibrosis; leaves open relevance to human arrhythmias.

Key Points

To examine whether functional intercellular gap junction coupling occurs between adult ventricular myocytes and myofibroblasts, and to determine how they bidirectionally affect each other's activity.
Co-cultured adult rabbit ventricular myocytes with pre-plated layers of cardiac myofibroblasts for 24 hours.
Assessed intercellular coupling via calcein dye transfer, connexin 43 (Cx43) immunostaining, and fluo-3 confocal calcium ([Ca2+]i) imaging during field stimulation.
Evaluated electrophysiological coupling and cellular responses using the gap junction inhibitor heptanol and the agonist sphingosine-1-phosphate (S-1-P).
Calcein transferred from myocytes to 52 ± 4% of surrounding myofibroblasts (n=8), which significantly declined to 12 ± 3% (n=6) following heptanol exposure.
Field stimulation of myocytes triggered [Ca2+]i increases in coupled myofibroblasts, an effect completely abolished by heptanol or myocyte absence.
Sphingosine-1-phosphate induced a quasi-linear current with a reversal potential near -40 mV in isolated myofibroblasts and depolarized co-cultured myocytes to approximately -20 mV without affecting myocytes cultured alone.

Structured PICO

Population

Co-cultured adult rabbit ventricular myocytes and cardiac myofibroblasts

Intervention

Gap junction uncoupler heptanol, field stimulation, and sphingosine-1-phosphate (S-1-P)

Comparator

Control conditions (absence of heptanol, absence of ventricular myocytes, or ventricular myocytes alone)

Outcome

Intercellular coupling measured by calcein dye transfer, Cx43 immunostaining, and intracellular Ca2+ transientssurrogate

This study demonstrates bi-directional gap junction-mediated interactions between ventricular myocytes and myofibroblasts, allowing myofibroblasts to influence myocyte electrical activity.

Cite This Study

Chilton et al. (2007) studied this question.

synapsesocial.com/papers/6a7cc1b152489361ef8ce774 https://doi.org/10.1113/jphysiol.2007.135038

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Evidence of intercellular coupling between co‐cultured adult rabbit ventricular myocytes and myofibroblasts

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Evidence of intercellular coupling between co‐cultured adult rabbit ventricular myocytes and myofibroblasts

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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