Tumor necrosis factor-alpha gene and protein expression in adult feline myocardium after endotoxin administration.
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Why the study?
Does endotoxin administration induce TNFa mRNA and protein expression in adult feline myocardium?
Population
Adult feline myocardium (cats weighing 3.4±0.2 kg, random sex) and isolated feline cardiac myocytes.
Comparison
Endotoxin administration. vs Diluent (Krebs-Henseleit buffer).
Design
Preclinical
Follow-up
Up to 210 minutes in vitro, 180 minutes in vivo
Authors
Discussion
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Implication
Endotoxin-induced myocardial TNFα synthesis may mediate cardiac depression in sepsis; leaves open translation from feline models to human therapy.
Structured PICO
Does endotoxin administration induce TNFa mRNA and protein expression in adult feline myocardium?
P
Population
Adult feline myocardium (cats weighing 3.4±0.2 kg, random sex) and isolated feline cardiac myocytes.
I
Intervention
Endotoxin administration (10 or 125 µg/ml in vitro, or 100 µg/kg boluses in vivo).
C
Comparator
Diluent (Krebs-Henseleit buffer).
O
Outcome
TNFa mRNA and protein biosynthesis in the myocardium.surrogate
This study provides the first evidence that adult mammalian myocardium, including cardiac myocytes, synthesizes biologically active TNFa in response to endotoxin, contributing to myocardial depression.
Cite This Study
Kapadia et al. (1995) studied this question.
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