What question did this study set out to answer?

To measure platelet activation status and agonist responsiveness using whole-blood flow cytometry in patients with coronary artery disease compared to age-matched and young healthy controls.

October 1, 1997Arteriosclerosis Thrombosis and Vascular BiologyOpen Access

Altered Platelet Function Detected by Flow Cytometry

View Full Paper

Ask AI

Bookmark

Why the study?

Does coronary artery disease alter platelet activation state and responsiveness to physiological agonists compared to healthy controls?

Key Points

To measure platelet activation status and agonist responsiveness using whole-blood flow cytometry in patients with coronary artery disease compared to age-matched and young healthy controls.
Analyzed peripheral blood from 65 patients with coronary artery disease (mean age 58 years; 54 male, 11 female), with a random subcohort of 12 males compared to 12 age-matched controls and 10 young male controls (mean age 25 years).
Utilized whole-blood flow cytometry to quantify basal and agonist-stimulated (ADP 0.1–10 µmol/L and thrombin 0.02–0.32 mu/mL) expression of bound fibrinogen, P-selectin, GPIb, and GPIIb-IIIa.
Basal P-selectin expression was significantly reduced in coronary artery disease patients compared with age-matched controls (P = .0005), whereas basal fibrinogen binding showed no significant difference.
Agonist-stimulated platelets in patients exhibited significantly decreased fibrinogen binding relative to age-matched controls (P < .03), though agonist-induced fibrinogen binding and P-selectin were highest in young controls (P < .05).
Surface expression of GPIb and GPIIb-IIIa was lowest in coronary artery disease patients with angina and highest in young control subjects.

Structured PICO

Does coronary artery disease alter platelet activation state and responsiveness to physiological agonists compared to healthy controls?

Population

65 patients with documented coronary artery disease (54 male, 11 female; mean age 58 years). A subgroup of 12 male patients (mean age 52 years) was compared with 12 age-matched male control subjects (mean age 52 years) and 10 normal young male subjects (mean age 25 years).

Comparator

Age-matched male control subjects and normal young male subjects

Outcome

Platelet activation status ex vivo and platelet responsiveness to physiological agonists in vitro (bound fibrinogen and expression of P-selectin, GPIb, and GPIIb-IIIa at rest and in response to ADP and thrombin)surrogate

Atherosclerosis in coronary artery disease is associated with impaired platelet aggregatory responses and degranulation compared to healthy controls, independent of age-related changes.

Discussion

No takes yet. Share an insight, caveat, or question.

Implication

CAD platelet hyporeactivity should not alter therapy; leaves open causality and mechanisms in case-control data.

Cite This Study

Knight et al. (1997) studied this question.

synapsesocial.com/papers/6a82ff21f71f34e1aae8de84 https://doi.org/10.1161/01.atv.17.10.2044

View Full Paper

Ask AI

Bookmark

Altered Platelet Function Detected by Flow Cytometry

Key Points

Structured PICO

Discussion

Implication

Cite This Study

Altered Platelet Function Detected by Flow Cytometry

Key Points

Structured PICO

Discussion

Implication

Cite This Study

Also Consider

Also Consider