Key result
PKG expression in cultured rat aortic vascular smooth muscle cells transformed them to a more contractile-like morphology and restored the capacity of cAMP/cGMP analogues to inhibit cell migration.
Population
Cultured rat aortic vascular smooth muscle cells (PKG-deficient cell lines)
Comparison
Transfection to express cyclic GMP-dependent… vs PKG-deficient cell lines
Design
Preclinical
Authors
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May inform VSMC-targeted therapies in vascular disease; leaves open translation from this in vitro model to in vivo or clinical settings.
PKG expression promotes a contractile-like phenotype in cultured vascular smooth muscle cells, indicating its role in preventing dedifferentiation.
Boerth et al. (2008) studied Vascular smooth muscle cell phenotype modulation. PKG expression (transfection of PKG-deficient cell lines) vs. PKG-deficient cell lines was evaluated on Phenotypic modulation and proliferation. PKG expression in cultured rat aortic vascular smooth muscle cells transformed them to a more contractile-like morphology and restored the capacity of cAMP/cGMP analogues to inhibit cell migration.
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