What question did this study set out to answer?

Determine whether 5-hydroxytryptamine administration alters pulmonary capillary blood volume by constricting pulmonary venules in anesthetized dogs.

March 1, 1963Journal of Applied Physiology

Pulmonary capillary blood volume in dog: effects of 5-hydroxytryptamine

View Full Paper

Ask AI

Bookmark

Why the study?

Does 5-hydroxytryptamine constrict pulmonary venules and increase pulmonary capillary blood volume in anesthetized dogs?

Population

Anesthetized dogs (n=14)

Comparison

5-hydroxytryptamine (5-HT) vs Baseline (pre-intervention measurements)

Design

Preclinical

Authors

RYRoscoe C. YoungMeharry Medical College HNHitoshi NaganoInstituto Tecnológico de Aeronáutica TVThomas R. VaughanUniversity of California, San Francisco

Discussion

Member takes

Implication

Hypothesis-generating for 5-HT effects on pulmonary capillaries; leaves open translation to human disease.

Key Points

Determine whether 5-hydroxytryptamine administration alters pulmonary capillary blood volume by constricting pulmonary venules in anesthetized dogs.
Measured pulmonary diffusing capacity in anesthetized dogs (N=14) using a positive-pressure single-breath carbon monoxide gas mixture with alveolar sampling at 10 seconds.
Calculated relative pulmonary capillary blood volume by measuring carbon monoxide diffusing capacity across multiple arterial oxygen tension levels before and after 5-hydroxytryptamine administration.
Pulmonary capillary blood volume increased by 6% to 133% in 10 of 14 dogs following 5-hydroxytryptamine infusion.
Systemic arterial pressure, left ventricular diastolic pressure, and airway pressure remained unchanged, and blood flow alterations did not correlate with capillary volume changes.

Structured PICO

Does 5-hydroxytryptamine constrict pulmonary venules and increase pulmonary capillary blood volume in anesthetized dogs?

Population

Anesthetized dogs (n=14)

Intervention

5-hydroxytryptamine (5-HT)

Comparator

Baseline (pre-intervention measurements)

Outcome

Changes in pulmonary capillary blood volume (Vc) measured via pulmonary diffusing capacity (Dl)surrogate

5-hydroxytryptamine causes pulmonary venous constriction, leading to passive congestion of the pulmonary capillary bed in a canine model.

Limitations

Cannot give absolute values for Vc since the rate at which CO replaces O2 in red cells in dogs is unknown

Cite This Study

Young et al. (1963) studied this question.

synapsesocial.com/papers/6a87ee71ba4ee383afbb5ab2 https://doi.org/10.1152/jappl.1963.18.2.264

View Full Paper

Ask AI

Bookmark

Pulmonary capillary blood volume in dog: effects of 5-hydroxytryptamine

Discussion

Member takes

Implication

Key Points

Structured PICO

Limitations

Cite This Study

Pulmonary capillary blood volume in dog: effects of 5-hydroxytryptamine

Discussion

Member takes

Implication

Key Points

Structured PICO

Limitations

Cite This Study

Also Consider

Also Consider