Why the study?
Does a rapid microplate-based enzymic assay reduce the time required to detect anti-hepatitis A virus neutralizing antibodies compared to standard in vitro assays?
Does a rapid microplate-based enzymic assay reduce the time required to detect anti-hepatitis A virus neutralizing antibodies compared to standard in vitro assays?
A novel microplate enzyme immunoassay significantly reduces the time required to detect anti-HAV neutralizing antibodies from 8 days to 3 days, which may improve virological diagnostics and vaccine evaluation.
May speed laboratory anti-HAV antibody detection; leaves open clinical validation and diagnostic adoption.
The slow growth of hepatitis A virus (HAV) in cell culture is one of the primary pitfalls in the development of sensitive and rapid methods for the detection and quantification of HAV and associated neutralizing antibodies. Currently, in vitro assays frequently require 8 days or more to detect and quantify the presence of HAV neutralizing antibodies. This study describes a rapid immunoassay that allowed the detection of anti-HAV neutralizing antibodies in only 3 days. This microplate-based enzymic assay may be applicable in virological diagnostics, in evaluating the immunogenicity of HAV vaccines and in quantifying neutralizing antibodies during the course of HAV infection.
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Azizi et al. (2009) studied this question.
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