What question did this study set out to answer?

To determine whether stimulation of muscarinic receptors coupled to the inhibitory GTP-binding protein Gi confers cardioprotection against ischaemic and reperfusion injury.

June 1, 1993Cardiovascular Research

Muscarinic receptor stimulation by carbachol improves functional recovery in isolated, blood perfused rabbit heart

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Why the study?

Does carbachol improve functional recovery in isolated, blood-perfused rabbit hearts subjected to ischaemia and reperfusion?

Population

Isolated, blood-perfused rabbit hearts subjected to 45 min zero flow normothermic ischaemia followed by 1 h…

Comparison

Muscarinic agonist carbachol with or without… vs Untreated control hearts with or without pacing…

Design

Preclinical

Follow-up

45 min ischaemia followed by 1 h reperfusion

Authors

MHMarc HendrikxHasselt University YTYoshihiro ToshimaUniversity of Occupational and Environmental Health Japan KMKanigula MubagwaUniversité Catholique de Bukavu

Discussion

Member takes

Implication

No immediate clinical implications; leaves open translation of muscarinic cardioprotection to human hearts.

Key Points

To determine whether stimulation of muscarinic receptors coupled to the inhibitory GTP-binding protein Gi confers cardioprotection against ischaemic and reperfusion injury.
Isolated blood-perfused rabbit hearts (Langendorff at 37°C) underwent 45 min of normothermic zero-flow ischaemia followed by 1 h of reperfusion across five experimental groups.
Hearts were assigned to untreated control (n=5), 10 µM carbachol (n=5), or 10 µM carbachol plus 5 µM atropine (n=5), all paced at 3 Hz except during ischaemia.
Two additional groups were paced continuously throughout ischaemia: control (n=5) and 10 µM carbachol (n=6).
In unpaced hearts, carbachol limited the rise in end-diastolic pressure during ischaemia to 21.6 (SEM 4.9) mm Hg compared to 57 (5.6) mm Hg in controls, and enhanced left ventricular pressure recovery at 60 min reperfusion (69.8 [4.9]% vs 29.3 [6.8]%).
In hearts paced throughout ischaemia, carbachol delayed contracture and preserved left ventricular pressure recovery at 46.7 (5.3)% versus 13.1 (8.4)% in paced controls, while reducing creatine kinase and lactate dehydrogenase enzyme release.
Post-reperfusion ATP recovery was significantly higher with carbachol in both unpaced (7.1 [0.72] vs 1.8 [0.58] µmol/g) and paced hearts (4.4 [0.77] vs 1.2 [0.39] µmol/g), an effect prevented by co-administration of atropine.

Structured PICO

Does carbachol improve functional recovery in isolated, blood-perfused rabbit hearts subjected to ischaemia and reperfusion?

Population

Isolated, blood-perfused rabbit hearts subjected to 45 min zero flow normothermic ischaemia followed by 1 h reperfusion (n=26)

Intervention

Muscarinic agonist carbachol (10 microM) with or without pacing during ischaemia

Comparator

Untreated control hearts with or without pacing during ischaemia

Outcome

Postischaemic functional recovery (left ventricular pressure, dP/dt, left ventricular end diastolic pressure)surrogate

Muscarinic receptor stimulation with carbachol improves functional recovery and confers cellular protection against ischaemia-reperfusion injury in isolated rabbit hearts.

Cite This Study

Hendrikx et al. (1993) studied this question.

synapsesocial.com/papers/6a8ae3c7bc51310b2bdebf89 https://doi.org/10.1093/cvr/27.6.980

Topics

Women and heart disease

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Muscarinic receptor stimulation by carbachol improves functional recovery in isolated, blood perfused rabbit heart

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Topics

Muscarinic receptor stimulation by carbachol improves functional recovery in isolated, blood perfused rabbit heart

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Topics

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