Key result
TMEV RNA isolated from the spinal cords of chronically infected mice is infectious, yielding at least 30-fold more infectious RNA than intact infectious virus.
Answers are grounded in this paper and cite their sources.
In a murine model of chronic TMEV infection, viral RNA isolated from the spinal cord is infectious and present at levels at least 30-fold higher than intact infectious virus.
The DA strain of Theiler's murine encephalomyelitis virus (TMEV) persistently infects cells of the spinal cord during the chronic phase of infection. Although in situ hybridization and reverse transcription-PCR have demonstrated the presence of viral RNA in the spinal cord, it has not been determined whether this RNA is infectious and, if so, how many PFU equivalents of virus the RNA can yield. In this study, we demonstrated that TMEV RNA isolated from the spinal cords of chronically infected mice is infectious and that there is at least 30-fold more infectious RNA than infectious virus in the spinal cords of these mice.
No takes yet. Share an insight, caveat, or question.
Libbey et al. (2007) studied Theiler's murine encephalomyelitis virus (TMEV) chronic infection (n=5). TMEV RNA isolation and transfection vs. Intact infectious viral particles (plaque assay of homogenate) was evaluated on Infectivity of TMEV RNA isolated from spinal cords (PFU/g) (30.4-fold increase). TMEV RNA isolated from the spinal cords of chronically infected mice is infectious, yielding at least 30-fold more infectious RNA than intact infectious virus.
Synapse has enriched 10 related papers. Consider them for comparative context: