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November 1, 1994British Journal of PharmacologyOpen Access

Identification of renal natriuretic peptide receptor subpopulations by use of the non‐peptide antagonist, HS‐142‐1

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Population

Rat (n=6), bovine (n=6), and human (n=6, male patients aged 40-66 years) kidney samples

Design

Preclinical

Authors

RRRichard A.D. RutherfordInstitute of Cytochemistry and Molecular PharmacologyYMYuzuru MatsudaTokyo Medical and Dental UniversityMWMartin R. WilkinsVascular Medicine

Discussion

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Implication

Rat glomerular NPRc predominance does not translate to humans; leaves open species-specific receptor validation before clinical translation.

Structured PICO

P
Population
Rat (n=6), bovine (n=6), and human (n=6, male patients aged 40-66 years) kidney samples
I
Intervention
In vitro autoradiography and competitive inhibition experiments using the specific guanylyl cyclase-linked receptor antagonist HS-142-1 and other selective ligands
O
Outcome
Distribution, relative density, affinity, and proportion of natriuretic peptide receptor subtypes in the kidneysurrogate

The study demonstrates significant species differences in renal natriuretic peptide receptor subtypes, showing that human and bovine glomeruli predominantly express guanylyl cyclase-linked receptors, unlike rats.

Limitations

  • The possibility of distinct binding to vascular endothelium could not be established due to the high density of binding sites on the medial layer and limited resolution of the autoradiographic images
  • Heterogeneity between species should be considered when selecting experimental models

Cite This Study

Rutherford et al. (1994) studied this question.

synapsesocial.com/papers/6a8b1912c716e89f35ded474https://doi.org/10.1111/j.1476-5381.1994.tb17082.x
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Expression and regulation of ANP receptor subtypes in rat renal glomeruli and papillae1989 · 59 citations
  2. 2HS-142-1, a Novel Non-peptide Antagonist for Atrial Natriuretic Peptide Receptor, Selectively Inhibits Particulate Guanylyl Cyclase and Lowers Cyclic GMP in LLC-PK1Cells1992 · 17 citations
  3. 3Immunohistochemical localization of atrial natriuretic peptide receptor in bovine kidney and lung.1989 · 27 citations
  4. 4Visualization of ANP receptor on glomeruli of bovine kidney by use of a specific antiserum1987 · 10 citations
  5. 5Physiological Role of Silent Receptors of Atrial Natriuretic Factor1987 · 940 citations