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March 1, 1986AJP Renal Physiology

Localization of atrial natriuretic peptide binding sites within the rat kidney

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Key result

High-affinity 125I-ANP binding sites were concentrated over glomeruli and arterial vasculature in rat kidneys, consistent with a glomerular filtration/hemodynamic mechanism for natriuresis.

Population

Rat kidney slices

Design

Preclinical

Authors

DHDennis P. HealyBayer (United States)DFDarrell D. FanestilUniversity of California, San Diego

Discussion

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Implication

Rat glomerular ANP binding supports hemodynamic natriuresis mechanism; leaves open human translation and therapeutic relevance.

Key Points

  • To identify and localize the specific renal binding sites of atrial natriuretic peptide to understand its diuretic and natriuretic mechanisms.
  • Incubated rat kidney sections with 150 pM iodine-125-labeled alpha-rat atrial natriuretic peptide (28 amino acids) alone or competitively displaced with 10 nM or 1 microM unlabeled peptide.
  • Conducted in vitro autoradiography using film and emulsion-coated coverslips alongside histochemical and immunohistochemical staining of discrete tubular segments.
  • High-affinity binding sites for atrial natriuretic peptide localized predominantly over glomeruli and renal arterial vasculature.
  • Lower-affinity binding sites were observed over proximal tubules and inner medullary collecting ducts, with no detectable binding in the remaining nephron segments.

Structured PICO

P
Population
Rat kidney slices
E
Exposure
Incubation with 150 pM 125I-ANP [alpha-rat ANP (alpha-rANP), 28 amino acids] alone or in the presence of 10 nM or 1 microM unlabeled ANP
O
Outcome
Localization of 125I-ANP binding sites by in vitro autoradiographysurrogate

The localization of high-affinity ANP binding sites in the rat kidney glomeruli suggests a glomerular filtration/hemodynamic mechanism for ANP-induced natriuresis and diuresis.

Cite This Study

Healy et al. (1986) studied this question. 125I-ANP was evaluated on Localization of 125I-ANP binding sites. High-affinity 125I-ANP binding sites were concentrated over glomeruli and arterial vasculature in rat kidneys, consistent with a glomerular filtration/hemodynamic mechanism for natriuresis.

synapsesocial.com/papers/6a99df2720f0e2865a45a55ehttps://doi.org/10.1152/ajprenal.1986.250.3.f573
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Localization of binding sites for alpha-rat atrial natriuretic polypeptide in rat kidney1986 · 83 citations
  2. 2In vitro autoradiographic localization of ANP receptors in rat kidney and adrenal gland1986 · 81 citations
  3. 3Autoradiographic localization of atrial natriuretic peptide receptor subtypes in rat kidney1990 · 20 citations
  4. 4Intrarenal Localization of Receptors for α-Rat Atrial Natriuretic Polypeptide: An Autoradiographic Study with [125I]-Labeled Ligand Injected in Vivo into the Rat Aorta1986 · 14 citations
  5. 5Autoradiographic discrimination of brain and atrial natriuretic peptide-binding sites in the rat kidney.1991 · 11 citations