What question did this study set out to answer?

To establish a relatively noninvasive, load-independent method for evaluating left ventricular contractility in living mice using echocardiography.

February 1, 1997AJP Heart and Circulatory Physiology

In vivo determination of left ventricular wall stress-shortening relationship in normal mice

View Full Paper

Ask AI

Bookmark

Population

28 anesthetized normal FVB/N mice

Comparison

Alteration of loading conditions by… vs Baseline state before alteration of myocardial…

Design

Preclinical

Authors

BHBrian D. HoitCardiac Imaging ZKZia U. KhanCentral South University CPCorinn M. Pawloski-DahmUniversity of Cincinnati

Discussion

Member takes

Implication

Supports noninvasive contractility assessment in murine models; leaves open clinical translation of stress-shortening relations.

Key Points

To establish a relatively noninvasive, load-independent method for evaluating left ventricular contractility in living mice using echocardiography.
Evaluated 28 anesthetized FVB/N mice using femoral artery cannulation and two-dimensional directed M-mode echocardiography with a 9-MHz transducer.
Manipulated loading conditions using intraarterial methoxamine (3–12 µg/g) to measure left ventricular fractional shortening across steady states.
Assessed changes in myocardial contractility after administering either intraperitoneal dobutamine (4 µg/g, n = 16) or verapamil (1–2 µg/g, n = 12).
Left ventricular systolic meridional stress and fractional shortening exhibited an inverse linear relationship at baseline (r = 0.80, slope = -0.19, intercept = 48%, standard error of estimate = 5.5%, P < 0.001).
Dobutamine induced an upward parallel shift in the stress-shortening relation (r = 0.87, slope = -0.21, intercept = 61%, standard error of estimate = 4.5%, P < 0.001).
Verapamil caused a downward shift in the stress-shortening relation (r = 0.48, slope = -0.05, intercept = 24%, standard error of estimate = 3.7%, P < 0.05), decreasing fractional shortening at matched stress levels.

Structured PICO

Population

28 anesthetized normal FVB/N mice

Intervention

Alteration of loading conditions by intraarterial methoxamine (3-12 microg/g) and alteration of myocardial contractility by either 4 microg/g intraperitoneal dobutamine (n = 16) or 1-2 microg/g verapamil (n = 12)

Comparator

Baseline state before alteration of myocardial contractility

Outcome

Left ventricular (LV) shortening fraction and its relation to LV systolic meridional stresssurrogate

Inverse stress-shortening relations can be assessed noninvasively in mice using echocardiography and are sensitive to alterations in inotropic state independent of loading conditions.

Cite This Study

Hoit et al. (1997) studied this question.

synapsesocial.com/papers/6a91b138ba3535a4ddf59559 https://doi.org/10.1152/ajpheart.1997.272.2.h1047

Topics

Echocardiography

View Full Paper

Ask AI

Bookmark

In vivo determination of left ventricular wall stress-shortening relationship in normal mice

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Topics

In vivo determination of left ventricular wall stress-shortening relationship in normal mice

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Topics

Also Consider