Passage of Classical Swine Fever Virus in Cultured Swine Kidney Cells Selects Virus Variants That Bind to Heparan Sulfate due to a Single Amino Acid Change in Envelope Protein E rns

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Population

Cultured swine kidney cells and Classical swine fever virus strains

Comparison

Heparinase I treatment, heparin, dextran… vs Untreated cells, unpassaged/minimally passaged…

Design

Preclinical

Authors

Discussion

Warrants caution in cell-culture adaptation of CSFV vaccines; leaves open effects on in vivo tropism and attenuation.

Population

Cultured swine kidney cells (SK6) and Classical swine fever virus (CSFV) strains (Brescia, clone C1.1.1, and clones isolated from infected pig blood)

Intervention

Heparinase I treatment, heparin, dextran sulfate, passage in SK6 cells, sequence analysis, replacement of E(rns) gene

Comparator

Untreated cells, unpassaged/minimally passaged virus clones

Outcome

Viral infection and binding to cellssurrogate

Passage of CSFV in cultured cells selects for variants that bind heparan sulfate due to a single amino acid mutation in the E(rns) envelope protein.

Hulst et al. (2000) studied this question.

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