Key result
The use of tandem IRES elements in a multicistronic vector proportionally increased the fluorescence output of GFP without significantly altering cap-dependent translation.
The use of tandem IRES elements in multicistronic vectors proportionally increases reporter gene fluorescence without disrupting endogenous gene regulation, enabling better analysis of weak promoters.
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Tandem IRES may optimize vector-based promoter analysis in animal models; leaves open translation to cardiovascular gene therapy applications.
Bouabe et al. (2008) studied this question. IRES-mediated polycistronic reporter system was evaluated on Fluorescence output of reporter genes (e.g., GFP). The use of tandem IRES elements in a multicistronic vector proportionally increased the fluorescence output of GFP without significantly altering cap-dependent translation.
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