What question did this study set out to answer?

To determine how insulin, palmitate, and acetate modulate endogenous myocardial triacylglycerol lipolysis and intracellular lipoprotein lipase activity in normal and ketotic diabetic states.

August 1, 1983Diabetes

Regulation of Triacylglycerol Lipolysis in the Perfused Hearts of Normal and Diabetic Rats

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Authors

VMVeeraraghavan K. MurthyUniversity of California, San Francisco MBMitchel D. BaumanMercy Medical Center Sioux City JSJoseph C. ShippUniversity of Nebraska at Omaha

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Implication

Experimental study reveals differential regulation of cardiac lipolysis by insulin and palmitate in diabetic rats, highlighting mechanisms of myocardial lipid accumulation.

Key Points

To determine how insulin, palmitate, and acetate modulate endogenous myocardial triacylglycerol lipolysis and intracellular lipoprotein lipase activity in normal and ketotic diabetic states.
Perfused isolated hearts from control and ketotic diabetic rats ex vivo for up to 120 minutes with or without glucose, physiological to high insulin (up to 500 μU/ml), and palmitate (0.5 mM).
Quantified triacylglycerol breakdown rates, residual lipid stores, and intracellular lipoprotein lipase activity across experimental conditions.
In fatty acid-free perfusate, diabetic hearts showed elevated rates and net amounts of lipolysis relative to controls, though residual triacylglycerol levels after 120 minutes were equivalent between groups.
Physiological insulin inhibited lipolysis in control hearts only when glucose was present, but failed to inhibit lipolysis in diabetic hearts even at supraphysiological levels up to 500 μU/ml.
Palmitate (0.5 mM) completely suppressed triacylglycerol lipolysis in both control and diabetic hearts regardless of glucose presence, selectively reducing intracellular lipoprotein lipase activity in diabetic tissue.

Cite This Study

Murthy et al. (1983) studied this question.

synapsesocial.com/papers/6a969d81d45ba51c86b2e6fb https://doi.org/10.2337/diab.32.8.718

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Implication

Experimental study reveals differential regulation of cardiac lipolysis by insulin and palmitate in diabetic rats, highlighting mechanisms of myocardial lipid accumulation.

Regulation of Triacylglycerol Lipolysis in the Perfused Hearts of Normal and Diabetic Rats

Discussion

Member takes

Implication

Key Points

Cite This Study

Regulation of Triacylglycerol Lipolysis in the Perfused Hearts of Normal and Diabetic Rats

Discussion

Member takes

Implication

Key Points

Cite This Study

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Also Consider