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August 1, 2000AJP Renal Physiology

Regulation of Ca2+-activated K+channels by multifunctional Ca2+/calmodulin-dependent protein kinase

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Key result

Inhibition of CAMKII with KN62 abolished or reduced to near zero the ANG II-induced BK(Ca) feedback response in mesangial cells.

Population

Mesangial cells

Comparison

KN62 or CAMKII with calmodulin plus ATP vs Absence of CAMKII inhibitor or CAMKII

Design

Preclinical

Authors

SSSteven C. SansomUniversity of Nebraska Medical CenterRMRong MaHeilongjiang University of Chinese MedicinePCPamela K. CarminesUniversity of Nebraska Medical Center

Discussion

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Implication

CAMKII mediates mesangial BK(Ca) responses to ANG II in rodents; leaves open any role in human glomerular disease.

Structured PICO

P
Population
Mesangial cells
I
Intervention
KN62 (specific inhibitor of CAMKII) or CAMKII with calmodulin plus ATP
C
Comparator
Absence of CAMKII inhibitor or CAMKII
O
Outcome
BK(Ca) channel activity and intracellular Ca(2+) concentrationsurrogate

CAMKII is involved in the BK(Ca) feedback response of mesangial cells to ANG II, potentiating the influence of increased intracellular Ca2+ on the channel.

Limitations

  • An additional effect of CAMKII to activate a voltage-gated Ca(2+) channel cannot be ruled out by these experiments.

Cite This Study

Sansom et al. (2000) studied this question. KN62 was evaluated on BK(Ca) feedback response. Inhibition of CAMKII with KN62 abolished or reduced to near zero the ANG II-induced BK(Ca) feedback response in mesangial cells.

synapsesocial.com/papers/6a96fd996b29a448da82519bhttps://doi.org/10.1152/ajprenal.2000.279.2.f283
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Ca-dependent facilitation of cardiac Ca current is due to Ca-calmodulin-dependent protein kinase1994 · 236 citations
  2. 2A new generation of Ca2+ indicators with greatly improved fluorescence properties.1985 · 21,778 citations
  3. 3Inactivation of calcium-activated chloride channels in smooth muscle by calcium/calmodulin-dependent protein kinase1997 · 108 citations
  4. 4Role of Potassium Channels in the Vascular Response to Endogenous and Pharmacological Vasodilators2008 · 64 citations
  5. 5Autophosphorylation and activation of Ca2+/calmodulin-dependent protein kinase II in intact nerve terminals.1988 · 83 citations