Key result
Curcumin-crosslinked acellular bovine pericardium demonstrated dramatically reduced calcium content and better mechanical, hemocompatible, and cytocompatible performance compared to glutaraldehyde fixation.
Why the study?
Glutaraldehyde-crosslinked pericardial tissues exhibit high cell toxicity and calcification accelerating bioprosthetic valve failure, motivating the search for alternative crosslinking reagents.
Does curcumin-crosslinked acellular bovine pericardium reduce calcification compared to glutaraldehyde crosslinking in preclinical models?
Does curcumin-crosslinked acellular bovine pericardium reduce calcification compared to glutaraldehyde crosslinking in preclinical models?
Answers are grounded in this paper and cite their sources.
Curcumin is a feasible crosslinking reagent for acellular bovine pericardium that inhibits calcification and improves mechanical and biocompatibility profiles in preclinical models, showing potential for bioprosthetic heart valves.
Glutaraldehyde (GA) crosslinked bovine or porcine pericardium tissues exhibit high cell toxicity and calcification in the construction of bioprosthetic valves, which accelerate the failure of valve leaflets and motivate the exploration for alternatives. Polyphenols, including curcumin, procyanidin and quercetin, etc, have showed great calcification inhibition potential in crosslinking collagen and elastin scaffolds. Herein, we developed an innovative phenolic fixing technique by using curcumin as the crosslinking reagent for valvular materials. X-ray photoelectron spectroscopy and Fourier transform infrared spectrometry assessments confirmed the hydrogen bond between curcumin and acellular bovine pericardium. Importantly, the calcification inhibition capability of the curcumin-crosslinked bovine pericardium was proved by the dramatically reduced Ca 2+ content in the curcumin-fixed group in in vitro assay, a juvenile rat subcutaneous implants model, as well as an osteogenic differentiation model. In addition, the results showed that the curcumin-fixed bovine pericardium exhibited better performance in the areas of mechanical performance, hemocompatibility and cytocompatibility, in comparison with the GA group and the commercialized product. In summary, we demonstrated that curcumin was a feasible crosslinking reagent to fix acellular bovine pericardium, which showed great potential for biomedical applications, particularly in cardiovascular biomaterials with calcification inhibition capacity.
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Liu et al. (2020) studied Bioprosthetic valve calcification. Curcumin-crosslinked acellular bovine pericardium vs. Glutaraldehyde (GA) crosslinked bovine pericardium and commercialized product was evaluated on Calcification inhibition (Ca2+ content), mechanical performance, hemocompatibility, and cytocompatibility. Curcumin-crosslinked acellular bovine pericardium demonstrated dramatically reduced calcium content and better mechanical, hemocompatible, and cytocompatible performance compared to glutaraldehyde fixation.
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