Key result
All 3 immunologic types of poliomyelitis virus were successfully propagated in monkey kidney and skin tissue grafted onto embryonated egg membranes without preliminary adaptation.
Demonstrates that poliomyelitis virus can be propagated in monkey tissues grafted onto chick embryos without prior adaptation.
May support expanded viral culture models; leaves open translation beyond this Level 5 animal study.
These studies have demonstrated that all 3 immunologic types of poliomyelitis virus may be propagated successfully in monkey kidney and skin tissue grafted onto embryonated egg membrane. No preliminary “adaptation” is necessary for virus multiplication in this system. Presence of virus is not limited to the grafted tissue but is found also in moderate concentration in the allantoic fluid, the CAM, and the embryo. However, this is probably due to spillage and dissemination from the grafts where virus growth apparently occurs. More precise knowledge is needed with reference to the exact sites of multiplication and the specific cells participating in this activity.
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Li et al. (1954) studied Poliomyelitis virus propagation. Monkey kidney and skin tissue grafted onto embryonated egg membrane was evaluated on Virus multiplication. All 3 immunologic types of poliomyelitis virus were successfully propagated in monkey kidney and skin tissue grafted onto embryonated egg membranes without preliminary adaptation.