January 1, 1991Journal of Cardiovascular Pharmacology
Differential Effect of Cyclic GMP on the Release of Endothelin-1 from Cultured Endothelial Cells and Intact Porcine Aorta
Key result
L-NMMA potentiates thrombin-stimulated ET-1 release in intact aorta, indicating NO downregulates ET-1 production.
Population
Intact porcine aorta and cultured porcine aortic endothelial cells
Comparison
L-NMMA and SIN-1, with or without thrombin… vs Basal conditions or absence of L-NMMA
Design
Preclinical
Authors
Discussion
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Implication
NO-ET-1 regulation differs in intact vs cultured vessels; leaves open translation to human endothelial dysfunction or therapy.
Structured PICO
P
Population
Intact porcine aorta and cultured porcine aortic endothelial cells
I
Intervention
L-NMMA (inhibition of NO release) and SIN-1, with or without thrombin stimulation
C
Comparator
Basal conditions or absence of L-NMMA
O
Outcome
Production/release of endothelin-1 (ET-1)surrogate
The study demonstrates that nitric oxide downregulates thrombin-stimulated endothelin-1 production in intact porcine aorta, a regulatory mechanism that appears lost in cultured endothelial cells.
Cite This Study
Boulanger et al. (1991) studied this question. L-NMMA was evaluated on Production of endothelin-1 (ET-1). L-NMMA potentiated thrombin-stimulated release of endothelin-1 in intact porcine aorta, but not in cultured porcine aortic endothelial cells, indicating NO downregulates ET-1 production in intact tissue.