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July 3, 2021Open Access

IGF-I up-regulates IGFBP-5 synthesis in aortic smooth muscle cells by boosting promoter activity ~3.5-fold.

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Population

Porcine aortic smooth muscle cells

Comparison

IGF-I vs control

Design

Preclinical in vitro laboratory study

Key result

IGF-I up-regulates IGFBP-5 synthesis by transcriptionally activating the IGFBP-5 gene in aortic smooth muscle cells, with the promoter being 3.5-fold more active in treated cells.

Authors

DCDavid R. ClemmonsNorthwestern UniversityTPTracy PrevetteUniversity of North Carolina at Chapel HillSHScott B. HawesUniversity of North Carolina at Chapel Hill

Discussion

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Overview

Hypothesis-generating in aortic smooth muscle cells; leaves open relevance to human vascular disease.

Structured PICO

P
Population
Porcine aortic smooth muscle cells (SMCs)
I
Intervention
Insulin-like Growth Factor-I (IGF-I)
C
Comparator
Control cells (untreated aortic SMCs and fibroblasts)
O
Outcome
IGFBP-5 synthesis and mRNA expression levelssurrogate

IGF-I specifically up-regulates IGFBP-5 synthesis in aortic smooth muscle cells via transcriptional activation, providing insight into vascular smooth muscle cell regulation.

Cite This Study

Clemmons et al. (2021) studied this question. Insulin-like Growth Factor-I (IGF-I) vs. Control cells was evaluated on IGFBP-5 synthesis and mRNA levels. IGF-I up-regulates IGFBP-5 synthesis by transcriptionally activating the IGFBP-5 gene in aortic smooth muscle cells, with the promoter being 3.5-fold more active in treated cells.

synapsesocial.com/papers/6aa5f89c3f91f0dc5278fcc6https://doi.org/10.17615/g544-p276
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Vascular smooth muscle cells synthesize two forms of insulin‐like growth factor binding proteins which are regulated differently by the insulin‐like growth factors1993 · 89 citations
  2. 2Evidence That IGF Binding Protein-5 Functions as a Ligand-Independent Transcriptional Regulator in Vascular Smooth Muscle Cells2004 · 92 citations
  3. 3Regulation of insulin‐like growth factor (IGF) binding protein‐2 synthesis and degradation by platelet‐derived growth factor and the IGFs is enhanced by serum deprivation in vascular smooth muscle cells1995 · 32 citations
  4. 4Insulin-like growth factor binding protein production by bovine and human vascular smooth muscle cells: production of insulin-like growth factor binding protein-6 by human smooth muscle.1996 · 18 citations
  5. 5Insulin Like Growth Factor Binding Protein-5 Regulates Excessive Vascular Smooth Muscle Cell Proliferation in Spontaneously Hypertensive Rats via ERK 1/2 Phosphorylation2013 · 17 citations