What does this research mean for the field?

Calcium ions are required for both mechanical contraction and electrical activity in the heart, with residual tissue calcium sustaining electrical activity during calcium-free perfusion unless chelated with citrate. Novelty: ClaimNovelty.INCREMENTAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

June 1, 1933Experimental Biology and Medicine

Electrical and Mechanical Changes in Isolated Heart Following Changes in Calcium Content of Perfusing Fluid

Q: What is the clinical evidence from this study?

Study design: Other. Population: Isolated rabbit heart. Intervention: Changes in calcium content and addition of sodium citrate in perfusing fluid vs. Solutions without calcium, solutions with citrate and no calcium. Primary outcome: Amplitude of contraction and electrical changes.

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Key result

Omitting calcium from perfusing fluid nearly halts contraction, while adding citrate stops all electromechanical activity.

Population

Isolated rabbit hearts

Comparison

Perfusing solutions with varying calcium concentrations and citrate vs standard reference solution

Design

Ex vivo physiological experiment

Authors

EBEmmet B. BayScripps Health FMFranklin C. McLeanUniversity of St Andrews AHA HastingsNational Association of School Nurses

Discussion

Member takes

Implication

Supports foundational role of calcium in isolated cardiac activity; leaves open translation to human in vivo physiology.

Structured PICO

Population

Isolated rabbit hearts perfused at 38°C to evaluate electrical and mechanical changes following changes in calcium content.

Intervention

Perfusion with solutions of varying calcium content, including omission of calcium and addition of sodium citrate

Comparator

Standard reference solution containing 0.7 millimol of calcium ions per liter and 0.8 millimol per liter of non-ionized calcium in the form of calcium citrate

Outcome

Amplitude of contraction and electrical changessurrogate

Calcium ions are necessary for both mechanical and electrical activity in the isolated rabbit heart, as demonstrated by complete cessation of activity when citrate is used to bind residual calcium.

Cite This Study

Bay et al. (1933) studied Isolated rabbit heart. Changes in calcium content and addition of sodium citrate in perfusing fluid vs. Solutions without calcium, solutions with citrate and no calcium was evaluated on Amplitude of contraction and electrical changes. Omission of calcium from the perfusing fluid caused almost total cessation of contraction, while adding citrate to calcium-free solutions caused all mechanical and electrical activity to cease.

synapsesocial.com/papers/6aa8699882a97bc6a7aa9322 https://doi.org/10.3181/00379727-30-6925

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Electrical and Mechanical Changes in Isolated Heart Following Changes in Calcium Content of Perfusing Fluid

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Electrical and Mechanical Changes in Isolated Heart Following Changes in Calcium Content of Perfusing Fluid

Discussion

Member takes

Implication

Structured PICO

Cite This Study

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Also Consider