Key result
Fetal aortic cultures sustain viral replication for up to 12 weeks without gross cytoarchitectural damage.
Why the study?
A human fetal aorta organ culture system with long-term viability and capacity to support viral replication was needed to study virus-aortic vessel interactions in vitro.
Human fetal aorta organ cultures can be maintained for prolonged periods and support viral replication without gross degenerative changes, providing a model for studying virus-aortic vessel interactions.
Answers are grounded in this paper and cite their sources.
Offers ex vivo model for virus-aorta studies; leaves open in vivo relevance and clinical implications.
Organ cultures were established with use of human fetal aorta obtained after death; cellular elements were preserved in the cultures for periods of up to eight weeks, with maintenance of their in vivo tissue relationships. Histologically, these cultures preserve the three coats of the aorta, including an intact endothelial layer cells. The aorta organ cultures supported the replication of echovirus 11, herpes simplex virus type 1, adenovirus 2, and coxsackievirus B5 without the production of detectable gross cytoarchitectural degenerative changes. Coxsackievirus B5 and adenovirus 2 were excreted by infected cultures for prolonged periods of up to 12 weeks. This human fetal aorta organ culture system, with its long-term viability and capacity to support viral replication, provides a useful model for in vitro study of virus-aortic vessel interactions.
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Blacklow et al. (1975) studied Human fetal aorta. Viral infection (echovirus 11, herpes simplex virus type 1, adenovirus 2, and coxsackievirus B5) was evaluated on Viral replication and cytoarchitectural changes. Human fetal aorta organ cultures supported the replication of multiple viruses for up to 12 weeks without producing detectable gross cytoarchitectural degenerative changes.
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