Supplementary Figure 4 from Aberrant CircTMEM45A Facilitates Inflammatory Progression of Esophageal Squamous Cell Carcinoma through m5C-Mediated NLRP3 Activation

Supplementary Figure 4. Validation of circTMEM45A overexpression and knockdown efficiencies. (A) Endogenous expression levels of circTMEM45A in esophageal squamous epithelial cells and five ESCC cell lines. (B) Empty vector frame pLC5-ciR for the construction of circTMEM45A overexpression vector. (C) Design of siRNAs targeting the backsplicing junction (BSJ) of circTMEM45A. (D-E) qRT-PCR analysis of circTMEM45A and TMEM45A mRNA expression in KYSE30 and TE1 cells transfected with the circTMEM45A overexpression vector. (F) qRT-PCR verification of circTMEM45A knockdown efficiency in KYSE150 cells. (G-H) qRT-PCR analysis of circTMEM45A and TMEM45A mRNA expression in KYSE150 and KYSE410 cells transfected with circTMEM45A siRNA-1, which was used in the following experiments. (I) Quantitative analysis of IHC staining in xenograft tumor samples. ns: not significant, **P<0.01, ***P<0.001.