Supplementary Figure 6 from Aberrant CircTMEM45A Facilitates Inflammatory Progression of Esophageal Squamous Cell Carcinoma through m5C-Mediated NLRP3 Activation

Supplementary Figure 6. Screening of downstream pathways of circTMEM45A. (A-C) Heatmap (A), volcano plot (B), and bar graph (C) highlight DEGs in circTMEM45A-silenced KYSE150 cells compared to control groups. (D) KEGG functional analysis of the DEGs. (E-F) qRT-PCR analysis of the expression of five mRNAs in the TNF pathway in circTMEM45A-overexpressing KYSE30 and TE1 cells. (G-H) qRT-PCR analysis of the expression of five mRNAs in the TNF pathway in circTMEM45A-silenced KYSE150 and KYSE410 cells. (I) qRT-PCR analysis of TNF and IL-18 mRNA expression in circTMEM45A-overexpressing KYSE30 cells and circTMEM45A-silenced KYSE150 cells. (J) qRT-PCR analysis of NLRP3, Caspase1, and GSDMD mRNA expression in circTMEM45A-overexpressing KYSE30 cells and circTMEM45A-silenced KYSE150 cells. (K) Western blot analysis of NLRP3, pro-caspase1, caspase1, GSDMD, and N-GSDMD protein levels in circTMEM45A-overexpressing KYSE30 cells and circTMEM45A-silenced KYSE150 cells. (L) LDH assay assessing pyroptosis changes in circTMEM45A-overexpressing KYSE30 cells and circTMEM45A-silenced KYSE150 cells. The relative gray value (target/GAPDH) is the average of all independent repeated experimental data (n = 3). ns: not significant, *P<0.05, **P<0.01, ***P<0.001.