Overexpression and knockdown of IL1B and IL1R1 impacts NLRP3 activation in esophageal squamous cell carcinoma, indicating a role for circTMEM45A.
Key Points
MAIN FINDING: CircTMEM45A facilitates NLRP3 activation, contributing to the inflammatory progression of esophageal squamous cell carcinoma.
KEY EVIDENCE: qRT-PCR and western blot analyses confirmed the significant overexpression and knockdown efficiency of IGF2BP2, HUR, IL1B, and IL1R1 in KYSE150 cells.
APPROACH: The study employed RAP experiments alongside qRT-PCR and western blot methods to verify gene expression levels.
SIGNIFICANCE: Understanding the role of circTMEM45A in cancer progression can lead to new therapeutic targets for esophageal squamous cell carcinoma.