Supplementary Figure 13 from Aberrant CircTMEM45A Facilitates Inflammatory Progression of Esophageal Squamous Cell Carcinoma through m5C-Mediated NLRP3 Activation

Supplementary Figure 13. The overexpression and knockdown efficiency verification of IGF2BP2, HUR, IL1B, and IL1R1. (A-B) RAP experiments detected the enrichment of circTMEM45A (A) and IL1R1 (B) by circTMEM45A probe. (C-F) qRT-PCR and western blot analyses confirmed the overexpression efficiency of IGF2BP2 (C-D) and HUR (E-F) at both mRNA and protein levels in KYSE150 cells. (G-J) qRT-PCR and western blot analyses confirmed the knockdown efficiency of IGF2BP2 (G-H) and HUR (I-J) at both mRNA and protein levels in KYSE150 cells. (K-N) qRT-PCR and western blot verified the knockdown efficiency of IL1B (K-L) and IL1R1 (M-N) at the mRNA and protein levels in KYSE150 cells. The relative gray value (target/GAPDH) is the average of all independent repeated experimental data (n = 3). ns: not significant, ***P<0.001.