Supplementary Figure 10 from Aberrant CircTMEM45A Facilitates Inflammatory Progression of Esophageal Squamous Cell Carcinoma through m5C-Mediated NLRP3 Activation

Supplementary Figure 10. Interaction between circTMEM45A and U2AF2 protein. (A) RAP assay showing the enrichment of the circTMEM45A probe for IL1B in KYSE150 cells. (B-D) qRT-PCR and western blot analyses of U2AF2 mRNA (B-C) and protein (D) expression in circTMEM45A-overexpressing KYSE30 cells and circTMEM45A-silenced KYSE150 cells. (E-F) qRT-PCR and western blot confirmed the knockdown efficiency of U2AF2 in KYSE150 cells. (G) qRT-PCR analysis of circTMEM45A expression after silencing U2AF2 in KYSE150 cells. The relative gray value (target/GAPDH) is the average of all independent repeated experimental data (n = 3). ns: not significant, **P<0.01, ***P<0.001.