Supplementary Figure S1 from The C-Terminal Kinase Domain–Binding and Suppression Motif Prevents Constitutive Activation of FGFR2

This is a FACS study (also classified as: Western Blotting).

Key Points

The study assesses GFPhi NMuMG cells to evaluate FGFR2 variants and their activation.
Isolation of cells involved using FACS and confirmation via western blotting techniques.
The analysis offers insights into the regulatory mechanisms preventing FGFR2 activation.
The methods enable a detailed understanding of FGFR2 interactions and functionality.

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Analysis uses FACS to isolate GFPhi NMuMG cells expressing FGFR2 variants, highlighting their activation control.

Isolation and analysis of GFPhi NMuMG cells expressing Fgfr2-GFP variants using FACS (includes gating strategy) and western blotting.

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Zingg et al. (2025) studied this question.

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