In vivo quantification of myocardial stiffness in hypertensive porcine hearts using MR elastography

PURPOSE: To determine alteration in left ventricular (LV) myocardial stiffness (MS) with hypertension (HTN). Cardiac MR elastography (MRE) was used to estimate MS in HTN induced pigs and MRE-derived MS measurements were compared against LV pressure, thickness and circumferential strain. MATERIALS AND METHODS: Renal-wrapping surgery was performed to induce HTN in eight pigs. LV catheterization (to measure pressure) and cardiac MRI (1.5 Tesla; gradient echo-MRE and tagging) was performed pre-surgery at baseline (Bx), and post-surgery at month 1 (M1) and month 2 (M2). Images were analyzed to estimate LV-MS, thickness, and circumferential strain across the cardiac cycle. The associations between end-diastolic (ED) and end-systolic (ES) MS and (i) mean LV pressure; (ii) ED and ES thickness, respectively; and (iii) circumferential strain were evaluated using Spearman's correlation method. RESULTS: From Bx to M2, mean pressure, MRE-derived stiffness, and thickness increased while circumferential strain decreased significantly (slope test, P ≤ 0.05). Both ED and ES MS had significant positive correlation with (i) mean pressure (ED MS: ρ = 0.56; P = 0.005 and ES MS: ρ = 0.45; P = 0.03); (ii) ED thickness ( ρ = 0.73; P < 0.0001) and ES thickness ( ρ = 0.84; P < 0.0001), respectively; but demonstrated a negative trend with circumferential strain (ED MS: ρ = 0.31 and ES MS: ρ = 0.37). CONCLUSION: This study demonstrated that, in a HTN porcine model, MRE-derived MS increased with increase in pressure and thickness. LEVEL OF EVIDENCE: 1 J. Magn. Reson. Imaging 2017;45:813-820.