November 1, 1973Journal of General Virology

Temperature-sensitive Poliovirus Mutants Defective in Repression of Host Protein Synthesis are also Defective in Structural Protein

Q: What is the clinical evidence from this study?

Study design: Other. Population: Poliovirus infection. Intervention: Temperature-sensitive (ts) mutations in structural genes vs. Wild-type poliovirus or non-structural gene mutants. Primary outcome: Repression of host cell protein synthesis (psr character).

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Key result

Temperature-sensitive poliovirus mutants with repressor defects in host protein synthesis were found to carry defects solely in structural protein genes.

Population

Poliovirus mutants (wild-type ts+ and ts mutants)

Comparison

Temperature shift to restrictive temperature… vs Permissive temperature and wild-type poliovirus…

Design

Preclinical

Authors

ASA. Steiner-PryorAustralian National University PCPaul D. CooperAustralian National University

Discussion

Member takes

Implication

Structural protein ts defects impair poliovirus host shutoff; leaves open repressor mapping in wild-type strains.

Structured PICO

Population

Poliovirus mutants (wild-type ts+ and ts mutants)

Exposure

Temperature shift to restrictive temperature (39.5 °C) with minimized gene expression (guanidine addition, cystine depletion)

Comparator

Permissive temperature (37 °C) and wild-type poliovirus / non-structural gene mutants

Outcome

Repression of host cell protein synthesissurrogate

Repression of host protein synthesis by poliovirus is dependent on the configuration of a product of the structural protein gene.

Cite This Study

Steiner-Pryor et al. (1973) studied Poliovirus infection. Temperature-sensitive (ts) mutations in structural genes vs. Wild-type poliovirus or non-structural gene mutants was evaluated on Repression of host cell protein synthesis (psr character). Temperature-sensitive poliovirus mutants with repressor defects in host protein synthesis were found to carry defects solely in structural protein genes.

synapsesocial.com/papers/6a657ca4a6c99762add5f422 https://doi.org/10.1099/0022-1317-21-2-215

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November 1, 1973Journal of General Virology

Temperature-sensitive Poliovirus Mutants Defective in Repression of Host Protein Synthesis are also Defective in Structural Protein

View Full Paper

Ask AI

Bookmark

Key result

Temperature-sensitive poliovirus mutants with repressor defects in host protein synthesis were found to carry defects solely in structural protein genes.

Population

Poliovirus mutants (wild-type ts+ and ts mutants)

Comparison

Temperature shift to restrictive temperature… vs Permissive temperature and wild-type poliovirus…

Design

Preclinical

Authors

ASA. Steiner-PryorAustralian National University PCPaul D. CooperAustralian National University

Discussion

Member takes

Implication

Structural protein ts defects impair poliovirus host shutoff; leaves open repressor mapping in wild-type strains.

Structured PICO

Population

Poliovirus mutants (wild-type ts+ and ts mutants)

Exposure

Temperature shift to restrictive temperature (39.5 °C) with minimized gene expression (guanidine addition, cystine depletion)

Comparator

Permissive temperature (37 °C) and wild-type poliovirus / non-structural gene mutants

Outcome

Repression of host cell protein synthesissurrogate

Repression of host protein synthesis by poliovirus is dependent on the configuration of a product of the structural protein gene.

Cite This Study

synapsesocial.com/papers/6a657ca4a6c99762add5f422 https://doi.org/10.1099/0022-1317-21-2-215

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

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