April 1, 1975Journal of General Virology

Poliovirus Temperature-sensitive Mutants Defective in Cytopathic Effects are also Defective in Synthesis of Double-stranded RNA

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Population

Poliovirus (wild-type and 20 temperature-sensitive mutants) in host cells

Comparison

Incubation at restrictive temperatures vs Incubation at permissive temperature and…

Design

Preclinical

Authors

DGD. J. GarwesMinistère de l'Agriculture, des Pêcheries et de l'Alimentation PWP.J. WrightMarine Scotland PCPaul D. CooperAustralian National University

Discussion

Member takes

Implication

May inform replicase-targeted antivirals; leaves open in vivo validation of dsRNA-driven cytopathic effects.

Structured PICO

Population

Poliovirus (wild-type and 20 temperature-sensitive mutants) in host cells

Intervention

Incubation at restrictive temperatures (39.2 to 39.6 degrees C)

Comparator

Incubation at permissive temperature (37 degrees C) and wild-type poliovirus

Outcome

Cytopathic effects (measured by trypan blue absorption) and synthesis of double-stranded RNAsurrogate

Late cytopathic effects of poliovirus are dependent on the replicase I gene product, likely double-stranded RNA, rather than the prevention of host macromolecule synthesis.

Cite This Study

Garwes et al. (1975) studied this question.

synapsesocial.com/papers/6a6ff37afebe604dd708c47a https://doi.org/10.1099/0022-1317-27-1-45

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Poliovirus Temperature-sensitive Mutants Defective in Cytopathic Effects are also Defective in Synthesis of Double-stranded RNA

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Poliovirus Temperature-sensitive Mutants Defective in Cytopathic Effects are also Defective in Synthesis of Double-stranded RNA

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider