Improved vectors for stable expression of foreign genes in mammalian cells by use of the untranslated leader sequence from EMC virus
Population
Mammalian cells (DHFR deficient and DHFR containing cells)
Comparison
Dicistronic mRNA expression vectors utilizing an… vs Previously described dicistronic expression…
Design
Preclinical
Authors
Discussion
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Member takes
Implication
May advance stable gene expression vectors in research; leaves open in vivo validation and clinical translation.
Structured PICO
P
Population
Mammalian cells (DHFR deficient and DHFR containing cells)
I
Intervention
Dicistronic mRNA expression vectors utilizing an internal ribosomal entry site from encephalomyocarditis (EMC) virus
C
Comparator
Previously described dicistronic expression vectors without the EMC virus leader sequence
O
Outcome
Stable high level expression of inserted cDNAs without rearrangement or deletion upon selectionsurrogate
The use of the EMC virus leader sequence in dicistronic vectors enables stable, high-level expression of foreign genes in mammalian cells without vector rearrangement.
Cite This Study
Kaufman et al. (1991) studied this question.