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July 1, 1992European Journal of Biochemistry

Dependence of the adenovirus tripartite leader on the p220 submit of eukaryotic initiation factor 4F during in vitro translation

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Population

In vitro translation model using reticulocyte lysate

Comparison

Translation of a reporter gene coupled to the… vs Translation of encephalomyocarditis virus RNA

Design

Preclinical

Authors

ATAdri A.M. ThomasUtrecht UniversityGSGert C. ScheperJohnson & Johnson (Israel)MKMiranda KELEIJNUtrecht University

Discussion

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Implication

Challenges poliovirus-based models of translation; leaves open p220 dependence across viral systems and clinical contexts.

Structured PICO

P
Population
In vitro translation model using reticulocyte lysate
I
Intervention
Translation of a reporter gene (chloramphenicol acetyltransferase) coupled to the adenovirus tripartite leader (TPT) 5'UTR, with p220 subunit degraded by foot-and-mouth-disease L-protease construct
C
Comparator
Translation of encephalomyocarditis virus RNA
O
Outcome
Dependence of TPT mRNA translation on intact p220 / eukaryotic initiation factor 4Fsurrogate

The study demonstrates that adenovirus TPT 5'UTR-directed translation is dependent on the p220 subunit of eukaryotic initiation factor 4F in vitro.

Cite This Study

Thomas et al. (1992) studied this question.

synapsesocial.com/papers/6a73a3051aa2fc9e78fb6d43https://doi.org/10.1111/j.1432-1033.1992.tb17073.x
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Potential secondary and tertiary structure in the genomic RNA of foot and mouth disease virus1987 · 100 citations
  2. 2Efficientin vitrosynthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter1984 · 6,458 citations
  3. 3Eukaryotic initiation factors-4E and -4F stimulate 5' cap-dependent as well as internal initiation of protein synthesis.1992 · 66 citations
  4. 4Adenovirus inhibition of cellular protein synthesis involves inactivation of cap-binding protein1991 · 177 citations
  5. 5Adenovirus late protein synthesis is resistant to the inhibition of translation induced by poliovirus.1987 · 33 citations