Generation of Antisera to Mouse Insulin-Like Growth Factor Binding Proteins (IGFBP)-1 to -6: Comparison of IGFBP Protein and Messenger Ribonucleic Acid Localization in the Mouse Embryo

Mouse embryos of 13.5 days post coitum, and liver and lung tissues of corresponding gene deletion (knock-out) mutant mice and wild-type litter mates.

Generation of antisera to synthetic peptide fragments of mouse IGFBP-1 through -6, evaluated via enzyme-linked immunosorbent assay, Western blotting, immunohistochemistry, and mRNA in situ hybridization.

Specificity of the produced antisera and tissue-specific expression patterns (protein and mRNA localization) of the six IGFBPs.surrogate