What question did this study set out to answer?

To determine how intact endothelium influences vascular contractile and relaxant responses to calcium channel agonists, potassium, noradrenaline, and calcium in rat thoracic aorta.

January 1, 1988Pharmacology & Toxicology

Influence of Endothelium on the Response to Calcium Agonists, Calcium, Potassium and Noradrenaline in Rat Aorta

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Population

Isolated thoracic aorta from rats

Comparison

Removal of endothelium and exposure to calcium… vs Aortic preparations with intact endothelium

Design

Preclinical

Authors

EMErich O. MikkelsenAarhus University GCGonzalo CostaUniversidad de la República de Uruguay NNNiels C. Berg NyborgNovo Nordisk (Denmark)

Discussion

Member takes

Overview

Endothelium-derived modulation of contractility in rat aorta warrants consideration in vascular experiments; leaves open translation to human disease or therapy.

Key Points

To determine how intact endothelium influences vascular contractile and relaxant responses to calcium channel agonists, potassium, noradrenaline, and calcium in rat thoracic aorta.
Evaluated isolated rat thoracic aortic rings with intact and denuded endothelium under exposure to BAY K 8644, CGP 28392, potassium, noradrenaline, and extracellular calcium.
Assessed concentration-dependent contractile responses (10⁻⁹–10⁻⁵ M) and relaxation in potassium-precontracted vessels at higher agonist concentrations (10⁻⁴ M).
Endothelial removal enhanced contractile responses to both BAY K 8644 and CGP 28392, while maintaining the higher relative contractile efficacy of BAY K 8644 over CGP 28392.
Endothelial denudation caused a leftward potency shift in concentration-response curves for potassium and noradrenaline without altering maximal contraction.
Vessel relaxation at high agonist concentrations (10⁻⁴ M) and calcium-induced contractions in activated rings were unaffected by the absence of endothelium.

Structured PICO

Population

Isolated thoracic aorta from rats

Intervention

Removal of endothelium and exposure to calcium agonists (BAY K 8644, CGP 28392), potassium, noradrenaline, and calcium

Comparator

Aortic preparations with intact endothelium

Outcome

Contractile and relaxant responses to the applied substancessurrogate

Endothelium-derived factors depress the contractile response to calcium agonists, potassium, and noradrenaline in rat aorta, likely by influencing smooth muscle cell membrane potential.

Cite This Study

Mikkelsen et al. (1988) studied this question.

synapsesocial.com/papers/6a87d7916841b83273ad2f95 https://doi.org/10.1111/j.1600-0773.1988.tb01838.x

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January 1, 1988Pharmacology & Toxicology

Influence of Endothelium on the Response to Calcium Agonists, Calcium, Potassium and Noradrenaline in Rat Aorta

View Full Paper

Ask AI

Bookmark

Population

Isolated thoracic aorta from rats

Comparison

Removal of endothelium and exposure to calcium… vs Aortic preparations with intact endothelium

Design

Preclinical

Authors

EMErich O. MikkelsenAarhus University GCGonzalo CostaUniversidad de la República de Uruguay NNNiels C. Berg NyborgNovo Nordisk (Denmark)

Discussion

Member takes

Overview

Endothelium-derived modulation of contractility in rat aorta warrants consideration in vascular experiments; leaves open translation to human disease or therapy.

Key Points

To determine how intact endothelium influences vascular contractile and relaxant responses to calcium channel agonists, potassium, noradrenaline, and calcium in rat thoracic aorta.
Evaluated isolated rat thoracic aortic rings with intact and denuded endothelium under exposure to BAY K 8644, CGP 28392, potassium, noradrenaline, and extracellular calcium.
Assessed concentration-dependent contractile responses (10⁻⁹–10⁻⁵ M) and relaxation in potassium-precontracted vessels at higher agonist concentrations (10⁻⁴ M).
Endothelial removal enhanced contractile responses to both BAY K 8644 and CGP 28392, while maintaining the higher relative contractile efficacy of BAY K 8644 over CGP 28392.
Endothelial denudation caused a leftward potency shift in concentration-response curves for potassium and noradrenaline without altering maximal contraction.
Vessel relaxation at high agonist concentrations (10⁻⁴ M) and calcium-induced contractions in activated rings were unaffected by the absence of endothelium.

Structured PICO

Population

Isolated thoracic aorta from rats

Intervention

Removal of endothelium and exposure to calcium agonists (BAY K 8644, CGP 28392), potassium, noradrenaline, and calcium

Comparator

Aortic preparations with intact endothelium

Outcome

Contractile and relaxant responses to the applied substancessurrogate

Endothelium-derived factors depress the contractile response to calcium agonists, potassium, and noradrenaline in rat aorta, likely by influencing smooth muscle cell membrane potential.

Cite This Study

Mikkelsen et al. (1988) studied this question.

synapsesocial.com/papers/6a87d7916841b83273ad2f95 https://doi.org/10.1111/j.1600-0773.1988.tb01838.x

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