What question did this study set out to answer?

To determine the host genetic mechanisms governing susceptibility or resistance to encephalomyocarditis virus (EMC-D)-induced diabetes in mice.

June 1, 1993Journal of General Virology

A genetically determined host factor controlling susceptibility to encephalomyocarditis virus-induced diabetes in mice

View Full Paper

Ask AI

Bookmark

Why the study?

Does genetic strain affect susceptibility to encephalomyocarditis virus-induced diabetes in mice?

Population

SJL/J male mice and C57BL/6J male mice

Comparison

Encephalomyocarditis (EMC)-D virus injection vs Comparison between susceptible and resistant…

Design

Preclinical

Follow-up

Up to 96 hours after injection

Authors

YKYup KangAjou University JYJi Won YoonBeth Israel Deaconess Medical Center

Discussion

Member takes

Implication

Genetic modulation of beta-cell viral receptors may determine murine diabetes susceptibility; leaves open extension to human viral diabetes pathogenesis.

Key Points

To determine the host genetic mechanisms governing susceptibility or resistance to encephalomyocarditis virus (EMC-D)-induced diabetes in mice.
Assessed pancreatic insulin mRNA and viral RNA dynamics at 0 to 96 hours following EMC-D viral injection in diabetes-susceptible SJL/J and diabetes-resistant C57BL/6J male mice.
Evaluated EMC-D viral binding capacity in both freshly isolated and cultured pancreatic beta cells across both mouse strains.
Pancreatic insulin mRNA in susceptible SJL/J mice decreased sharply by 24 hours post-infection to trace levels at 72 hours, whereas levels remained stable through 96 hours in resistant C57BL/6J mice.
Viral RNA peaked at 48 hours post-injection in SJL/J pancreata but was undetectable in C57BL/6J mice except for low levels at 24 and 48 hours.
EMC-D bound readily to fresh SJL/J beta cells but scarcely to fresh C57BL/6J beta cells, though C57BL/6J beta cells expressed equivalent binding capacity after 24 hours in culture.

Structured PICO

Does genetic strain affect susceptibility to encephalomyocarditis virus-induced diabetes in mice?

Population

SJL/J male mice (susceptible to encephalomyocarditis virus-induced diabetes) and C57BL/6J male mice (resistant)

Intervention

Encephalomyocarditis (EMC)-D virus injection

Comparator

Comparison between susceptible (SJL/J) and resistant (C57BL/6J) mouse strains

Outcome

Insulin mRNA levels and EMC-D viral RNA levels in pancreatasurrogate

Genetic susceptibility to EMC-D virus-induced diabetes in mice appears to be controlled by the modulation of viral receptor expression on pancreatic beta cells in vivo.

Cite This Study

Kang et al. (1993) studied this question.

synapsesocial.com/papers/6a8e7b6034f2e8bd0f1d675a https://doi.org/10.1099/0022-1317-74-6-1207

View Full Paper

Ask AI

Bookmark

Implication

Genetic modulation of beta-cell viral receptors may determine murine diabetes susceptibility; leaves open extension to human viral diabetes pathogenesis.

A genetically determined host factor controlling susceptibility to encephalomyocarditis virus-induced diabetes in mice

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

A genetically determined host factor controlling susceptibility to encephalomyocarditis virus-induced diabetes in mice

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider