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December 1, 1986AJP Cell Physiology

Determinants of Ca2+-dependent stress maintenance in skinned swine carotid media

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Key result

In skinned swine carotid media, maintained stress magnitude depended on initial Ca2+ levels, with half-maximal responses at 1.4 x 10(-6) M for rapid development and 3.1 x 10(-7) M for maintenance.

Population

Detergent-skinned swine carotid media fibers

Comparison

Contraction with 0.82, 1.3, 2.1, or 7.2 microM… vs Different initial [Ca2+] levels and single vs…

Design

Preclinical

Authors

RMR. S. MorelandUniversity of VermontRMRichard A. MurphyUniversity of Southern California

Discussion

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Implication

Initial Ca2+ sets maintenance stress in skinned fibers; leaves open latch mechanisms and in vivo translation.

Structured PICO

P
Population
Detergent-skinned swine carotid media fibers
I
Intervention
Contraction with 0.82, 1.3, 2.1, or 7.2 microM Ca2+ followed by exposure to a lower [Ca2+]
C
Comparator
Different initial [Ca2+] levels and single vs cumulative [Ca2+] exposures
O
Outcome
Magnitude and Ca2+ sensitivity of stress maintenance and myosin light chain (MLC) phosphorylation levelssurrogate

In skinned swine carotid media, the magnitude of Ca2+-dependent stress maintenance is dependent on the initial activating Ca2+ concentration.

Limitations

  • Time- and stress-related deterioration of the preparation

Cite This Study

Moreland et al. (1986) studied this question. Ca2+ concentration was evaluated on Ca2+-dependent stress maintenance and myosin light chain phosphorylation. In skinned swine carotid media, maintained stress magnitude depended on initial Ca2+ levels, with half-maximal responses at 1.4 x 10(-6) M for rapid development and 3.1 x 10(-7) M for maintenance.

synapsesocial.com/papers/6a939cb550daee6a3ee1c186https://doi.org/10.1152/ajpcell.1986.251.6.c892
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Length-Tension Relationship of Smooth Muscle of the Hog Carotid Artery1973 · 311 citations
  2. 2Contraction of detergent-treated smooth muscle.1978 · 80 citations
  3. 3Myosin light chain phosphorylation associated with contraction in arterial smooth muscle1981 · 243 citations
  4. 4Calcium-Dependent Stress Maintenance Without Myosin Phosphorylation in Skinned Smooth Muscle1983 · 147 citations
  5. 5Activation of contraction and ATPase activity in intact and chemically skinned smooth muscle of rat portal vein. Dependence on Ca++ and muscle length.1983 · 33 citations