Concentration of autoantibodies to native 60‐kd ro/ss‐a and denatured 52‐kd ro/ss‐a in eluates from the heart of a child who died with congenital complete heart block
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Why the study?
Do autoantibodies to native 60-kd and denatured 52-kd Ro/SS-A specifically concentrate in the heart tissue of a child with congenital complete heart block?
Population
Tissues from a single stillborn child with congenital complete heart block, born to a 30-year-old mother…
Comparison
Acid elution of tissues and measurement of IgG… vs Eluates from brain, kidney, and skin; maternal…
Design
Case_report
Authors
Discussion
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Implication
Hypothesis-generating for anti-Ro/SS-A in congenital heart block; leaves open causal role and generalizability beyond this case.
Structured PICO
Do autoantibodies to native 60-kd and denatured 52-kd Ro/SS-A specifically concentrate in the heart tissue of a child with congenital complete heart block?
P
Population
Tissues (heart, skin, kidney, brain) from a single stillborn child (died at 34 weeks gestation) with congenital complete heart block (CCHB), born to a 30-year-old mother with systemic lupus erythematosus (SLE) and anti-Ro/SS-A antibodies.
I
Intervention
Acid elution of tissues and measurement of IgG and antibody titers (anti-native 60-kd and denatured 52-kd Ro/SS-A) by ELISA.
C
Comparator
Eluates from brain, kidney, and skin; maternal serum.
O
Outcome
Serologic specificity of acid eluates (enrichment of antibodies to native 60-kd and denatured 52-kd Ro/SS-A).surrogate
Autoantibodies to native 60-kd Ro/SS-A specifically concentrate in the heart tissue of a child with congenital complete heart block, suggesting a major role in its immunopathogenesis.
Limitations
- Single case study
- Does not rule out the involvement of antibodies to La/SS-B and/or the 52-kd Ro/SS-A in other cases of CCHB
- Does not explain why CCHB occurs in only a small number of children born to mothers who have anti-Ro/SS-A
Cite This Study
Reichlin et al. (1994) studied this question.
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